Assay of sequences using amplified genes

Abstract From the Canadian patent

A method for amplifying specific target nucleic acid sequences in a sample, the method comprising (a) contacting under hybridization conditions (i) two different immobilized or immobilizable primer nucleic acids, with (ii) a test sample containing target nucleic acid sequences, (b) contacting the resultant product of step (a) with an extender enzyme and at least one nucleic acid residue under conditions to elongate the resultant hybridized primer nucleic acids, and, (c) denaturing the product of step (b) to, produce immobilized target nucleic acid sequences and, (d) repeating at least once a cycle of steps (a), (b) and (c) with the product of step (c) of the previous cycle being used in …