Human cell line and triomas, antibodies, and transformants derived therefrom

Abstract From the Canadian patent

A stable, continuous human cell line or progeny thereof is produced that is resistant to 6-thioguanine and ouabain secretes less, than 40 ng/ml of endogenous IgM antibodies and grows with a doubling, time of about 18 hours. The cell line which preferably is adapted to, serum-free medium may be used as a fusion partner with an antibodyproducing cell line so as to generate antibodies. In addition it may, be electroporated with a vector containing a gene of interest to produce a transformed cell line which generates a protein encoded by the gene, such as an IgG or IgM antibody.