Aminoluciferin derivatives, processes for the production thereof and their application in the determination of enzyme activities

Abstract From the Canadian patent

The invention relates to D-aminoluciferin derivatives of the general formula I, (I), wherein, R1 represents a L-amino acid or peptide radical having up to 10 L-amino acid moieties said radical being bound via the (terminal) carboxyl group in the form of an amide and the free amino group thereof is optionally be protected by a common protecting group, or wherein R1 represents a monosaccharide or disaccharide radical. Said aminoluciferin derivatives can be cleaved by enzymes giving free aminoluciferin which can be determined luminometrically. The invention relates also to the use of said aminoluciferin derivatives for determining enzyme activities as well to a process for producing said …