Nucleic acid hybridization technique and kit therefor

Abstract From the Canadian patent

A novel assay method is suitable for detecting a polynucleotide in a liquid sample. The method comprises the steps of: (a) combining the sample with at least two different probes, being a first probe and a second probe to, form a reaction mixture, each probe comprising a singlestranded polynucleotide which contains a nucleotide sequence complementary to a portion of the target polynucleotide the, probes together forming hybrid molecules by complementary base pairing with the target polynucleotide neither probe, being affixed to a solid carrier the probes do not, hybridize with each other and the second probe having a, detectable label; (b) subsequently contacting the reaction mixture with a …