Process to induce desiccation tolerance in somatic embryos

Abstract From the Canadian patent

A process for inducing desiccation tolerance in an in vitro formed plant embryo to enable production of viable artificial seeds is disclosed. The process comprises culturing in vitro plant embryos stimulating, the embryos to proceed through globular and a heart shaped stage into an elongate-torpedo shaped stage and early cotyledon stage, inducing the embryos as early as the torpedo-shaped stage with a source of abscisic acid at an effective concentration of abscisic acid and for a sufficient period of time to cause expression of desiccation tolerance which includes change in cellular metabolism, electron transport processes and oxidationreduction reactions in the embryos and drying the …