Monoclonal antibody specific for a mammary tumor cell surface antigen

Abstract From the Canadian patent

A monoclonal antibody is produced which is specific for a cell surface antigen found predominantly on human mammary tumor cells. Animals are inoculated with human mammary tumor cells or cell material and, spleen cells obtained from the animals are fused with myeloma cells to produce hybridomas. The hybridomas are cultured as monoclones, and antibodies obtained from the individual clones are tested for their reactivity with several human mammary tumor cell lines and mammary tumor tissues. A clone which produces an antibody that reacts generally with human mammary tumor cells but not other tumors, such as mesotheliomas, is selected for further culturing to produce the antibody, and the …