Insulin analogues and process for their preparation

Abstract From the Canadian patent

Novel rapid-acting human insulin analogues are provided having less tendency to self-association into dimers tetramers, hexamers, or polymers. The novel human insulin analogues are ormed by substituting one or more of the amino acid residues of human insulin with naturally occuring amino acid residues. The amino acid residue substitutions are preferably more hydrophilic than the natural amino acid residue at the respective position in the molecule. Furthermore, the insulin analogues have the same charge or a greater negative charge at neutral pH than that of human insulin. Preferred amino acid substitutions are Asp Glu, Ser, Thr, His, and Ile, and more preferred substitutions are Asp and …