Polypeptide and protein products, and process for their production and use

Abstract From the Canadian patent

A process for the production of a fusion protein comprising an active portion of a chloramphenicol acetyltransferase (CAT) protein and a polypeptide. The fusion protein may be purified using CAT substrate affinity chromatography. The eucaryotic polypeptide may be calcitonin or a derivative thereof such as calcitonin-glycine. Other polypeptides described include enzymes such as chymosin prochymosin and preprochymosin, hormones such as ACTH insulins, and growth hormones and antigenic polypeptides such as foot and mouth disease virus antigenic polypeptide. The fusion protein may be cleaved at a site susceptible to selective enzymic or chemical cleavage to produce free polypeptide. The fusion …