Immobilized enzyme preparation and its use

Abstract From the Canadian patent

A stabilised enzyme preparation comprising an immobilised hydantoinase is useful for the preparation of a D(-)(optionally substituted phenyl)glycine or N-carbamoyl derivative thereof by hydrolysis of a 5-(optionally substituted phenyl)hydantoin. The enzyme preparation comprises cells or treated cells of a hydantoinase - producing organism immobilised within or on a suitable solid support, or an enzyme derived from the organism adsorbed on or bonded to a polymeric support, together with divalent metal ions which stabilise the preparation and render it capable of re-use. Processes for preparing the enzyme preparation and for using it to prepare commercially important end products such as …