Production of phage-associated lysin for diagnosis of streptococcal infection

Abstract From the Canadian patent

A process is provided herein for producing a lysin-free phage inoculum. The process includes first inoculating a growing Streptococcal culture with phage. The culture is incubated for a plurality of lytic cycles of phage until the cells are completely lysed to obtain a lysate. Finally cell debris and free lysin are removed from the lysate to form a lysin-free phage suitable for use as an inoculum. The advantages of the new process for phage production are three-fold. The amount of phage required to generate a new batch of phage inoculum is greatly reduced and the number of phage produced per initial phage is improved 104 fold. In addition, the phage lysate used as inoculum for phage …