Assaying method for primary amines using aromatic dialdehydes

Abstract From the Canadian patent

Primary amines are assayed by providing a substituted or unsubstituted naphthalene-2,3-dicarboxyaldehyde reactive with primary amines, reacting the substituted or unsubstituted naphthalene-2,3-dicarboxyaldehyde with at least one primary amine analyte and cyanide ion to form an adduct amenable to fluorometric or electrochemichal assaying techniques and fluorometrically or electrochemically assaying said adduct. The method according to the invention enables assaying of trace concentrations of analytes containing one or more primary amino groups.