Layered immunoassay using antibodies bound to transport particlesto determine the presence of an antigen

Abstract From the Canadian patent

A device is provided for determining the presence of an antigen which comprises a trapping zone which contains material capable of capturing free flowing enzyme linked antibodies, but not antibodies bound to a transport particle which flows freely through the trapping zone into the substrate zone and a, substrate zone which contains material capable of reacting with enzyme-linked antibodies to produce a reaction which indicates the presence of antibodies. A method of determining the presence of an antigen is provided wherein a sample is mixed with two classes of antibodies which are specific for the antigen being tested for, but which react with different antigen domains, wherein the …