Sequencing of peptides

Abstract From the Canadian patent

A peptide is sequenced from its carboxyl terminal by coupling with an isothiocyanate having a chemical formula R(X)Si(SCN)y where x=0-3 and y=1-4. R may be an alkyl or an aryl. The coupling reaction is preferably conducted at a temperature range of 50°C-90°C for a period to approximately sixty (60) minutes or less. A weak acid anhydride, preferably a carboxylic acid anhydride, or a weak acid chloride preferably, a carboxylic acid chloride may be mixed with the, isothiocyanate. An organic base catalyst selected from a group consisting of tertiary amines including aromatic amines such as pyridine may be utilized.