Solution-phase single hybridization assay for detecting polynucleotide sequences

Abstract From the Canadian patent

A process for determining the presence of a particular nucleic acid sequence in a test sample comprising, (a) chemically modifying nucleic acids in the test sample either to introduce a label or a reactive site in a manner that supports their hybridizability (b) contacting under hybridization conditions the chemically modified sample nucleic acids with a hybridizable nucleic acid probe which either when the, sample nucleic acids have been modified to introduce a label, carries a reactive site or, when the sample nucleic acids have been modified to introduce a reactive site is, labeled, (c) contacting the solution resulting from step (b) with a immobilized form of a reactive partner to the …