Quantitative determination of nucleic acid molecules and the reagent kitused

Abstract From the Canadian patent

The invention is related to the quantification of certain nucleic acid molecules, particularly the degree of amplification of genes and/or corresponding messenger RNA molecules using the sandwich or solution hybridization method, and the reagent kit used. The determination is carried out by comparing the number of the test nucleic acid molecules potentially present in several copies in given unit to the number of chosen standard nucleic acid molecules advantageously present in a constant number per same unit.