Methods for providing internal references for use in analyte-receptor assays

Abstract From the Canadian patent

A method is disclosed for providing internal references for use in an analyte-receptor assay for the, determination of a target analyte in a sample. The assay uses a signal producing system and an analyte receptor capable of binding the target analyte at a discrete test zone on a solid phase, in an amount directly proportional to the amount of target analyte in the sample. The method comprises binding a reference receptor, selected to bind with an analyte receptor conjugate capable of complexation with the target analyte, at a discrete reference zone on a solid phase. The reference receptor is selected such that the rates of binding of the analyte receptor conjugate to the reference …