Automatic process for purifying physiologically active substance, apparatus for the same, and carrier with proteinaceous ligand fixed theretoto be used for the same

Abstract From the Canadian patent

An automatic process for isolating and purifying physiologically active substances contained in large quantities of sample solutions, which comprises the following three steps: (1) filtration (a step for removing microbial cells and other insoluble matters) (2) ultrafiltration (a step for removing impurity having a molecular weight lower than that of the active substance, and concentrating the sample solution), and (3) affinity chromatography using a carrier with a substance having specific affinity to the active substance fixed thereto (a step for isolating pure product by selective adsorption and elution), an apparaturs for use in the same and a carrier employed in the same.