Method for locating and purifying dna containing single base mismatches
CA 1286618 C · Owner: Casna, Nancy J. · Inventors: Ford, John P.; Casna, Nancy J.; Novack, David F.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
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A method for distinguishing fragments of DNA which contain single base mismatches from their perfectly paired homologues is disclosed. Single stranded regions within a duplex fragment are modified with carbodiimide which reacts with unpaired guanine (G) and thymine (T) residues in DNA. Linear duplex DNA molecules do not react while DNA molecule with single base mismatches react quantitatively with carbodiimide. Following reaction with carbodiimide, the DNA molecules are fractionated on high percentage polyacrylamide gels such that modified and unmodified fragments can be clearly distinguished. Application of this technique in order to locate and purify DNA sequence differences responsible …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Granted July 23, 1991. A Canadian patent filed before October 1, 1989 lasted 17 years from grant, so its term ended July 23, 2008.
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Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.