Immobilized c-peptide antibody, process for preparation of and immunoassay using the same

Abstract From the Canadian patent

An antibody immobilized on a water-insoluble solid support by a heterobifunctional cross-linking reagent wherein said antibody is a, partially reduced form of immunogloblin being specific to human proinsulin C-peptide and having thiol groups is disclosed. The solid, support has amino groups and the cross-linking reagent is capable of forming two bonds, one with the thiol group and the other with the amino group. The antibody is useful in conducting immunoassay for proinsulin C-peptide in body fluid. Purification of an antiserum specific to human proinsulin C-peptide by means of immunoaffinity chromatography on 2 column carrying human proinsulin C-peptide or its fragment as ligand and the …