Solid phase diffusion assay

Abstract From the Canadian patent

A method is disclosed for the quantitative or qualitative determination of an analyte in a test sample comprising the steps of:, (a) binding a first substance to an insoluble porous sheet support said first substance being selected from the group consisting of ligands and receptors which bind said analyte, said support being covered by a layer of water impermeable material having a hole therein; (b) applying an aliquot of said test sample to said insoluble support through said hole; (c) permitting said test sample to diffuse through said insoluble support carrying said first substance; (d) applying a second substance to said insoluble support before, together with, or after application of …