Anticoagulating substance, process for preparing same and anticoagulant comprising same as an effective component

Abstract From the Canadian patent

Novel human placenta-derived anticoagulating substances having the following properties: (1) molecular weight of 34,000 ? 2,000 determined by SDS-polyacrylamide gel electrphoresis under reduced state;, (2) isoelectric point of 4.7 ? 0.1 determined by isoelectric column electrophoresis using an ampholyte; (3) stability, inactivated by heat treatment at 50°C for 30 minutes, stable at a pH of 4 - 10, and stable in plasma at 37°C for 30 minutes;, (4) activity, capable of prolonging a recalcification time a prothrombin time, and an activated partial thromboplastin time; and, (5) the existence of several amino acids including aspartic acid, threonine, serine, and so on; are prepared by …