Fluorogenic dihydroquinolone and dihydrocoumarin indicators for hydrogen peroxide

Abstract From the Canadian patent

Fluorogenic indicator compounds responsive to the presence of hydrogen peroxide and methods and test compositions for determining hydrogen peroxide in a test sample. Preferred indicator compounds are 3,4-dihydrocoumarin derivatives and 3,4-dihydro-2quinolone derivatives, especially those having a dialkylamino group at the 7-position. Such particularly preferred compounds are of the formula: wherein R1 is hydrogen, cyano, or -COOR6 -CONHR6 or, -CON(R6)2 where R6 is hydrogen alkyl, alkenyl, or, aryl; R3 and R4 are lower alkyl; and Y is =O or =N-R5 where R5 is hydrogen or lower alkyl. The indicator compounds yield highly fluorescent products upon oxidation by hydrogen peroxide in the presence …