Rotating culture chamber for uniform dispersal and culturing of cells or plant tissues

Abstract From the Canadian patent

A process and apparatus for culturing cells or plant tissues, comprising steps of (1) packing a culturing vessel with a mixture system consisting of a liquid medium and anchorage-independent cells, plant tissues or carrier, particles having adhered thereto anchorage-dependent cells, so that the culturing vessel is substantially filled with the mixture system, and (2) rotating the culturing vessel on its own substantially horizontal axis to, rotate the mixture system in the steady state synchronously with the culturing vessel, thereby moving the anchorageindependent cells, the plant tissues or the carrier particles having adhered thereto anchorage-dependent cells in the mixture system, to …