Urokinase zymogen and composition containing the same

Abstract From the Canadian patent

There is provided a zymogen of plasminogen activator which is produced by culturing human kidney cells in a culturing medium and recovering from the culture fluid by affinity chromatography using a column of immobilized anti-zymogen antibody. The zymogen is a polypeptide having a single chain with a molecular weight of about 50,000. Although the zymogen itself little exhibits plasminogen activator activity it mani-, fests a significant plasminogen activator activity by catalytic amounts of plasmin. The zymogen has a thrombolytic ability as high as three times that of a human urinary urokinase, and the zymogen is usable for treating human thrombus. The zymogen can be stabilized by the …