Monoclonal antibody for human hematopoietic glycoproteins and method

Abstract From the Canadian patent

This invention concerns a new monoclonal antibody (mAb) 4C, recognizing a specific antigen, Leu 200 found in human, hematopoietic tissues. The monoclonal antibody precipitates a series of glycoproteins (Leu 200) with a molecular weight range of about 190,000 to 230,000 from both T- and B-cell lines. The series of glycoproteins resolves into four discrete glycoprotein bands, the distribution of which varies according to the cell lines. Bands 3 and 4 predominate in a majority of T-cells whereas band 2 predominates in B cells. Thus, a Leu 200 antigen subset distribution is possible with mAb 4C. Bands 1 2, 3, 4 have, apparent molecular weights of 230K 215K, 205K, and 90K, respectively, with …