Method for determining lipid bound sialic acid in whole blood

Abstract From the Canadian patent

Abstract of the Disclosure, The amount of lipid bound sialic acid in a whole blood sample may be determined by a method, which may be automated involving the following steps: adding to the sample a lower alkyl alcohol and deionized distilled water; mixing the resulting admixture; adding to it a mixture of lower alkyl chlorinated hydrocarbon and a lower alkyl alcohol; treating by mixing and centrifuging the mixture until a recoverable substantially clear upper phase forms; recovering the upper phase and adding to it a protein-precipitating agent pre-, ferably in admixture with an adsorbing material; mixing the resulting admixture; recovering the resulting precipitate suspending the …