Process for the preparation of oligonucleotides

Abstract From the Canadian patent

The invention relates to a process for the preparation of oligonucleotides by the following steps: reaction of a nucleoside with a phosphine derivative reaction of the nucleotide derivative thus obtained with a nucleoside bonded to a polymeric carrier oxidation of, the carrier-bound nucleoside-nucleotide thus obtained with formation of phosphotriester groups blocking, of free primary 5'-OH groups elimination of a protec-, tive group from the terminal 5'-OH group where approp-, riate single or multiple repetition of the abovementioned steps to introduce further nucleoside phosphate or oligonucleoside phosphate units, and cleavage of the nucleoside-carrier bond and, where appropriate …