Purified plasminogen activator, process for its production and thrombolytic composition containing it

Abstract From the Canadian patent

A plasminogen activator obtained from a tissue cultured liquor of human normal tissue derived cells e.g. human embryonic derived cells, human placenta derived cells etc. and having the following properties: a) molecular weight measured by gel filtration: 63,000 + 10,000, b) isoelectric point : 7.0 - 8.5 c) affinity to fibrin: present, d) affinity to concanavalin A: present e) optimum pH. 7 - 9.5, and, f) stability : does not lose activity at 60°C for 10 hours but loses about 5% of activity at pH 2-3 at 98°C for a minute; a process for the production of the aforesaid plasminogen activator; and a thrombolytic composition containing the aforesaid plasminogen activator as an active ingredient …