Lipoprotein lipase suppression by endotoxin-induced mediator (shock assay)

Abstract From the Canadian patent

Mediator substances exhibiting inhibitory effect upon anabolic enzyme activity in mammals, are prepared by a method comprising gathering a sample of macrophage cells from a mammal in-, cubating a portion of the macrophage cells with a stimulating material that is associated with an invasive event and incu-, bating the macrophage cells to produce the mediator substance. The stimulator materials for example, may include endotoxins, trypanosomes and the like and the enzymes that may be obser-, ved range from lipoprotein lipase acetyl Coenzyme A carboxy-, lase and fatty acid synthetase to the inducers for red blood, cells growth and differentiation. The mediator substance may be utilized to …