Supports for use in synthesis of polynucleotides and apparatus for use in making such supports

Abstract From the Canadian patent

Disclosed herein is an automated apparatus for producing a modified silica gel, comprising: a) first valve means selectively, connectable to a supply of active reagents or wash solvents;, b) second valve means selectively connectable to a supply of nucleoside-active phosphite triester or a wash solvent;, c) third valve means selectively connectable to a supply of cleavage reagents or a wash solvent;, d) a collector valve for receiving the outflow from, respectively, said first second and, third valve means;, e) a closed flow circuit including a selector valve; a column containing a modified silica gel matrix; and pump means arranged intermediate said selector valve and said column in series …