Chromogenic and/or fluorogenic substrates for monitoring catalytic or enzymatic activity

Abstract From the Canadian patent

Novel substrates for monitoring of catalytic activity resulting in hydrolytic release of products which can be readily detected by spectrophotometric or fluorometric means are provided. The substrates have the general formula: wherein B is a nucleotide base capable of assisting in hydrolysis of the phosphate ester at the 3'-position; R is a moiety selected from the group consisting of umbelliferonyl 4-methyl-, umbelliferonyl, 3-flavonyl, o-nitrophenyl m-nitrophenyl, p-nitrophenyl, dinitrophenyl cyanophenyl, acylphenyl, carboxy-, phenyl phenylsulfonate, phenylsulfonyl and phenylsulfoxide; R' is a moiety selected from the group consisting of hydrogen alkyl, alkenyl cycloalkyl, aryl, araalkyl …