Method and reagent combination for the diagnosis of microorganisms

Abstract From the Canadian patent

A microbial diagnostic method based on the hybridization of nucleic acids on a solid carrier and a, reagent combination for carrying out the method. The method which can be used to identify all the microbes or microbial groups from a single sample containing nucleic acids of the microbes or microbial groups to be diagnosed after having first rendered said nucleic acids singlestranded, basically consists in adding to the sample two nucleic acid reagents for each microbe or microbial group to be diagnosed. One of these nucleic acid reagents consists of a nucleic acid fragment from the same microbe or microbial group, which is attached to a solid carrier in a single stranded form. The other …