Radio-immunoassay method for determining bupropion and active metabolites thereof and a test kit for use in the assay method

Abstract From the Canadian patent

An assay method of determining the concentration of bupropion and any active metalbolites thereof in a body fluid comprises (a) mixing together antisera comprising bupropion -specific antibodies, a labelled competitor of bupropion capable of competing with bupripion for binding to said antisera and body fluid, (b) mixing together a concentration of a standard amount of bupropion antisera and, labelled competitor, and (c) measuring the portion of labelled competitor either bound or unbound to the antibodies in each of step (a) and step (b) and comparing the results for step (a) with the results for step (b); the method is useful in determining the presence of bupropion a pharmacologically, …