Limulus lysate turbidity test for pyrogens
CA 1060323 A · Owner: Worthington Biochemical Corporation · Inventors: Teller, Joseph D.; Kelly, Kristine M.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
A sample of an aqueous liquid e.g. an aqueous parenterally-, administered drug is tested for determining the amount of any endotoxin contained therein by admixing a sample of the liquid with lysate obtained by water lysing washed amebocyte cells from the blood of the horseshoe crab (Limulus polyphemus) said lysate being buffered, at a pH between about 6.5 and about 6.8 and being at a concentration such that 1 ml thereof is reactive with 0.1 ml of an endotoxin solution containing 1 ng per ml of endotoxin to develop upon incubation for 60 minutes at 37°C turbidity exhibiting absorbancy between about 0.07 and about 0.2 at 360 nm, incubating the resulting admixture under temperature and time …
Read the whole patent
Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Granted August 14, 1979. A Canadian patent filed before October 1, 1989 lasted 17 years from grant, so its term ended August 14, 1996.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.